Journal: Biology of reproduction

Article Title: Impairment of the interleukin system in equine endometrium during the course of endometrosis.

doi: 10.1095/biolreprod.113.109447

Figure Lengend Snippet: FIG. 1. Representative pictures of immunohistochemical staining for IL1a (IL-1a; A–C), IL1b (IL-1b; D–F), IL6 (IL-6; G–I), IL1RI (IL-1RI; J–L), ILRII (IL-RII; M–O), and IL6Ra (IL-6a; P, R, S) in endometrial tissue in category I (first column; no degenerative changes) and category III (second and third column; severe endometrosis) endometrium during the estrous cycle. The negative controls are shown in T, U, and V. GE, glandular epithelium; GN, glandular nests; LE, luminal epithelium; S, stromal cells. Bar ¼ 100 lm (original magnification 320 for first and second column) and 50 lm (original magnification 340 for third column).

Article Snippet: Briefly, the sections were incubated with primary goat polyclonal antibodies against IL1a (anti-mouse; 15 lg/ml; #AF-400NA; R&D Systems), IL1b/IL1F2 (anti-equine; R&D System; #AF3340; 10 lg/ml), IL6 (anti-equine; 10 lg/ml; #AF1886; R&D Systems), IL6Ra (anti-mouse; 15 lg/ml; #AF1830; R&D Systems); IL1RI (anti-mouse; 5 lg/ml; #AF771; R&D Systems); IL1RII (antimouse; 15 lg/ml; #AF563; R&D Systems).

Techniques: Immunohistochemical staining, Staining

Journal: Biology of reproduction

Article Title: Impairment of the interleukin system in equine endometrium during the course of endometrosis.

doi: 10.1095/biolreprod.113.109447

Figure Lengend Snippet: FIG. 6. IL6 (IL-6) mRNA transcription (A) and the density of the immunohistochemical reaction (B; relative units; image analysis; Micro- Image) during the estrous cycle in Kenney category I (no degenerative changes), II, and III in equine endometrium (n ¼ 48). a,bStatistical differences between phases of the estrous cycle in Kenney category I. n,o,pStatistical differences between phases of the estrous cycle in Kenney category II. x,y,zStatistical differences between phases of the estrous cycle in Kenney category III. Asterisks indicate statistical differences between IL6 mRNA transcription and the density of the immunohistochemical reaction during the course of endometrosis within each phase of the estrous cycle (*P , 0.05; **P , 0.01; ***P , 0.001).

Article Snippet: Briefly, the sections were incubated with primary goat polyclonal antibodies against IL1a (anti-mouse; 15 lg/ml; #AF-400NA; R&D Systems), IL1b/IL1F2 (anti-equine; R&D System; #AF3340; 10 lg/ml), IL6 (anti-equine; 10 lg/ml; #AF1886; R&D Systems), IL6Ra (anti-mouse; 15 lg/ml; #AF1830; R&D Systems); IL1RI (anti-mouse; 5 lg/ml; #AF771; R&D Systems); IL1RII (antimouse; 15 lg/ml; #AF563; R&D Systems).

Techniques: Immunohistochemical staining

Journal: Biology of reproduction

Article Title: Impairment of the interleukin system in equine endometrium during the course of endometrosis.

doi: 10.1095/biolreprod.113.109447

Figure Lengend Snippet: FIG. 8. The influence of IL1a (IL-1a; 10 ng/ml), IL1b (IL-1b; 10 ng/ml), and IL6 (IL-6; 10 ng/ml) on PGE2 in vitro secretion from midluteal phase endometrial explants. The endometrial explants were assigned to category I (A), II (B), and III (C) according to Kenney. All values are expressed as n- fold change from the control. Asterisks indicate statistical differences between the groups (*P , 0.05; ***P , 0.001).

Article Snippet: Briefly, the sections were incubated with primary goat polyclonal antibodies against IL1a (anti-mouse; 15 lg/ml; #AF-400NA; R&D Systems), IL1b/IL1F2 (anti-equine; R&D System; #AF3340; 10 lg/ml), IL6 (anti-equine; 10 lg/ml; #AF1886; R&D Systems), IL6Ra (anti-mouse; 15 lg/ml; #AF1830; R&D Systems); IL1RI (anti-mouse; 5 lg/ml; #AF771; R&D Systems); IL1RII (antimouse; 15 lg/ml; #AF563; R&D Systems).

Techniques: In Vitro, Control

Journal: Biology of reproduction

Article Title: Impairment of the interleukin system in equine endometrium during the course of endometrosis.

doi: 10.1095/biolreprod.113.109447

Figure Lengend Snippet: FIG. 9. The influence of IL1a (IL-1a; 10 ng/ml), IL1b (IL-1b; 10 ng/ml), and IL6 (IL-6; 10 ng/ml) on in vitro PGF2a secretion from midluteal phase endometrial explants. The endometrial explants were assigned to category I (A), II (B), and III (C) according to Kenney. All values are expressed as n- fold change from the control. Asterisks indicate statistical differences between the groups (*P , 0.05; **P , 0.01).

Article Snippet: Briefly, the sections were incubated with primary goat polyclonal antibodies against IL1a (anti-mouse; 15 lg/ml; #AF-400NA; R&D Systems), IL1b/IL1F2 (anti-equine; R&D System; #AF3340; 10 lg/ml), IL6 (anti-equine; 10 lg/ml; #AF1886; R&D Systems), IL6Ra (anti-mouse; 15 lg/ml; #AF1830; R&D Systems); IL1RI (anti-mouse; 5 lg/ml; #AF771; R&D Systems); IL1RII (antimouse; 15 lg/ml; #AF563; R&D Systems).

Techniques: In Vitro, Control

Journal: Biology of reproduction

Article Title: Impairment of the interleukin system in equine endometrium during the course of endometrosis.

doi: 10.1095/biolreprod.113.109447

Figure Lengend Snippet: FIG. 10. The influence of IL1a (IL-1a; 10 ng/ml), IL1b (IL-1b; 10 ng/ml), and IL6 (IL-6; 10 ng/ml) on PTGS-2 mRNA transcription in midluteal phase endometrial explants incubated in vitro. The endometrial explants were assigned to category I (A), II (B), and III (C) according to Kenney. Asterisks indicate statistical differences between the groups (*P , 0.05; **P , 0.01; ***P , 0.001).

Article Snippet: Briefly, the sections were incubated with primary goat polyclonal antibodies against IL1a (anti-mouse; 15 lg/ml; #AF-400NA; R&D Systems), IL1b/IL1F2 (anti-equine; R&D System; #AF3340; 10 lg/ml), IL6 (anti-equine; 10 lg/ml; #AF1886; R&D Systems), IL6Ra (anti-mouse; 15 lg/ml; #AF1830; R&D Systems); IL1RI (anti-mouse; 5 lg/ml; #AF771; R&D Systems); IL1RII (antimouse; 15 lg/ml; #AF563; R&D Systems).

Techniques: Incubation, In Vitro

Journal: Biology of reproduction

Article Title: Impairment of the interleukin system in equine endometrium during the course of endometrosis.

doi: 10.1095/biolreprod.113.109447

Figure Lengend Snippet: FIG. 11. The influence of IL1a (IL-1a; 10 ng/ml), IL1b (IL-1b; 10 ng/ml), and IL6 (IL-6; 10 ng/ml) on PGES mRNA transcription in midluteal phase endometrial explants incubated in vitro. The endometrial explants were assigned to category I (A), II (B), and III (C) according to Kenney. Asterisks indicate statistical differences between the groups (*P , 0.05; **P , 0.01; ***P , 0.001).

Article Snippet: Briefly, the sections were incubated with primary goat polyclonal antibodies against IL1a (anti-mouse; 15 lg/ml; #AF-400NA; R&D Systems), IL1b/IL1F2 (anti-equine; R&D System; #AF3340; 10 lg/ml), IL6 (anti-equine; 10 lg/ml; #AF1886; R&D Systems), IL6Ra (anti-mouse; 15 lg/ml; #AF1830; R&D Systems); IL1RI (anti-mouse; 5 lg/ml; #AF771; R&D Systems); IL1RII (antimouse; 15 lg/ml; #AF563; R&D Systems).

Techniques: Incubation, In Vitro

Journal: Biology of reproduction

Article Title: Impairment of the interleukin system in equine endometrium during the course of endometrosis.

doi: 10.1095/biolreprod.113.109447

Figure Lengend Snippet: FIG. 12. The influence of IL1a (IL-1a; 10 ng/ml), IL1b (IL-1b; 10 ng/ml), and IL6 (IL-6; 10 ng/ml) on PGFS mRNA transcription in midluteal phase endometrial explants incubated in vitro. The endometrial explants were assigned to category I (A), II (B), and III (C) according to Kenney. Asterisks indicate statistical differences between the groups (*P , 0.05; **P , 0.01; ***P , 0.001).

Article Snippet: Briefly, the sections were incubated with primary goat polyclonal antibodies against IL1a (anti-mouse; 15 lg/ml; #AF-400NA; R&D Systems), IL1b/IL1F2 (anti-equine; R&D System; #AF3340; 10 lg/ml), IL6 (anti-equine; 10 lg/ml; #AF1886; R&D Systems), IL6Ra (anti-mouse; 15 lg/ml; #AF1830; R&D Systems); IL1RI (anti-mouse; 5 lg/ml; #AF771; R&D Systems); IL1RII (antimouse; 15 lg/ml; #AF563; R&D Systems).

Techniques: Incubation, In Vitro

Journal: Adipocyte

Article Title: Effects of SPARCL1 on the proliferation and differentiation of sheep preadipocytes

doi: 10.1080/21623945.2021.2010901

Figure Lengend Snippet: Primer sequences for a quantitative real-time polymerase chain reaction

Article Snippet: IL6 , Rabbit polyclonal antibody , 1:1000 , Bioss, bs-4587 R.

Techniques: Sequencing

Journal: Adipocyte

Article Title: Effects of SPARCL1 on the proliferation and differentiation of sheep preadipocytes

doi: 10.1080/21623945.2021.2010901

Figure Lengend Snippet: The information of antibodies used in Western blot

Article Snippet: IL6 , Rabbit polyclonal antibody , 1:1000 , Bioss, bs-4587 R.

Techniques: Western Blot

Journal: Adipocyte

Article Title: Effects of SPARCL1 on the proliferation and differentiation of sheep preadipocytes

doi: 10.1080/21623945.2021.2010901

Figure Lengend Snippet: SPARCL1 affects the Wnt/β-catenin pathway related genes (a) GSK3β, FZD8, Wnt10b, β-catenin, IL6 expression patterns in preadipocyte differentiation; These genes expression is the highest at day 6(** P < 0.01). (b) Inhibition and overexpression with the SPARCL1 gene affect the mRNA expression of GSK3β, FZD8, Wnt10b, β-catenin, IL6 . (c) Inhibition and overexpression with the SPARCL1 gene affect the protein expression of GSK3β, FZD8, Wnt10b, β-catenin, IL6. (d) Densitometric analyses of the Western blots. The first set of data in the figure is named ‘a’. Lowercase letters different from a represent significant data differences between groups ( P < 0.05), named ‘b, c, and d’ in that order, and the same lowercase letters represent non-significant data differences( P > 0.05)

Article Snippet: IL6 , Rabbit polyclonal antibody , 1:1000 , Bioss, bs-4587 R.

Techniques: Expressing, Inhibition, Over Expression, Western Blot